Inexpensive and simple set-up for field inversion gel electrophoresis

Pulsed-field gel electrophoresis.

DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...

Pulsed-field Gel Electrophoresis for Salmonella Infection

To detect Salmonella clusters, public health laboratories perform pulsed-field gel electrophoresis (PFGE) that provides a PFGE pattern, or DNA fingerprint. If the PFGE patterns of isolates from >2 persons are indistinguishable, the responsible bacteria may be related to a common source (1–3). PulseNet is a network of public health laboratories coordinated by the Centers for Disease Control and ...

Markers for Pulsed-field Gel Electrophoresis.

Field inversion gel electrophoresis as a typing system for group A streptococcus.

Field inversion gel electrophoresis (FIGE) was performed on 139 isolates of group A streptococcus (GAS) representing 72 different M types. Multiple isolates were tested for 27 M types (range, 2-19). Each of the M types tested had at least one unique banding pattern that was not observed in any other M type. For 23 of 27 M types for which multiple isolates were available, band profiles of the is...

Detection of early and late stage apoptosis with field inversion gel electrophoresis.

were picked onto MM plates and grown as before for 24 h. Replica plates were overlaid with nitrocellulose. One filter was blotted as described above (Figure 2a) and for the other, the nitrocellulose was removed after 10 min (Figure 2b). There is a clear titration in the strength of the ECL signal with the dilution of the TCR α-chain expressing cells. Without blotting, the signal was indistingui...